Separation and Analysis Methods

Occurrence: 2022/23 - 2S

Courses

Hours Actually Taught

LBBA_1

Learning outcomes of the curricular unit (knowledge, skills and competences to be developed by the students)

Acquiring knowledge and developing skills that enable students to interpret the sequences of operations of a biological process in steady states, in terms of separation processes, according to block diagrams or simple flow diagrams with and without recycling, as well as perform mass balances to separation processes applied to the extraction, purification and quantification of bioproducts in order to know the flows of matter circulating in the process. Know the fundamentals, limitations and applications of instrumental methods most commonly used in qualitative and quantitative analysis of bioproducts and food.

It is envisaged that students develop skills in linking theoretical and experimental knowledge that will enable them to perform the main separation processes, select and perform the main instrumental techniques of analysis.

Syllabus

1- Steady-state mass balances for unreacted processes. 2- Separation methods: solid-liquid separation: filtration, sedimentation, centrifugation. Liquid-liquid separation: extraction, distillation. Chromatography. Membrane processes. 3- Purification methods: Concentration. Crystallization. Drying. 4- Instrumental Methods of Analysis: optical methods, rheological methods, spectrophotometric methods, chromatographic methods.
Practical work: Separation and analysis methods applied to the recovery and purification of biomolecules and food processing. 

Demonstration of the syllabus coherence with the curricular unit's learning objectives

The subject aims to acquire knowledge and develop skills that enable students to understand the fundamentals of mass transfer and identify the main separation processes applied to the extraction, purification and quantification of bioproducts. Students will be provided with knowledge about the main separation processes applied to the recovery of biomolecules, such as proteins, lipids, pigments, as well as those related to food processing. The integration and adequacy of instrumental methods of analysis will allow the characterisation and quantification of bioproducts, providing students with a thorough knowledge on analytical methodologies.  

Teaching methodologies (including evaluation)

The classes are of a theoretical-practical nature where the subject will be exposed and practical work will be carried out in the scope of recovery and purification of biomolecules and food processing.

Demonstration of the coherence between the teaching methodologies and the learning outcomes

In theoretical sessions are introduced the fundamental concepts of general chemistry. In theoretical-practical sessions students will do exercises to apply the concepts presented in theoretical sessions. In laboratorial sessions will apply the concepts acquired in theoretical sessions.

Bibliography (Mandatory resources)

Azevedo, E. & Alves, A. (2009). Engenharia de Processos de Separação. Ensino da Ciência e da Tecnologia, IST Press, Portugal.
Bourne, (2002). Food Texture and Viscosity: concept and measurement, Academic press.
Geankoplis, C., Lepek, D., Hersel, A. (2018). Transport Processes and Separation Process, Pearson New International Edition, 5th Edição, United States.
Hunter_Lab, (2012) CIE L*a*b* Colour Scale.
Lima, N. and Mota, M. (2003). Biotecnologia ¿ Fundamentos e Aplicações, Lidel Edições Técnicas Lda, Portugal.
Michael, L. and Fikret, K. (2015). Bioprocess Engineering: Basic Concepts. 2nd ed. Pearson. United States.
Pinho, M.N. and Prazeres, D.M. (2008). Fundamentos de Transferência de Massa, Ensino da Ciência e da Tecnologia IST Press, Portugal.
Pombeiro A. (2003). Técnicas e operações unitárias em química laboratorial -Fundação Calouste Gulbenkian, 4ª ed., Portugal.